biotinylated anti crth2  (Miltenyi Biotec)

Journal: Clinical and Experimental Immunology

Article Title: CRTH2 expression on T cells in asthma

doi: 10.1111/j.1365-2249.2010.04161.x

Figure Lengend Snippet: (a) Representative fluorescence activated cell sorter (FACS) dot plots showing association of chemokine receptor homologous molecule expressed on T helper type 2 (Th2) lymphocytes (CRTH2) with CD3, CD4 and CD8, and CD45RO. The gates were set with reference to the isotype control for each antibody. There was no difference in the degree of staining (mean fluorescence) on CRTh2-positive T cells from asthmatics and controls. (b) Scatter-plot of the percentages of CRTH2 + T cells in blood of normal subjects (•) and asthmatics (▴). Bars represent the mean. No significant difference in the percentage of CRTH2 + T cells was seen between the groups.

Article Snippet: PBMC and BAL cells were incubated with biotinylated anti-CRTH2 mAb (Mitenyi Biotec, Bergisch Gladbach, Germany) at 4°C while suspended in PBS and 0·5% BSA.

Techniques: Fluorescence, Staining

Journal: Clinical and Experimental Immunology

Article Title: CRTH2 expression on T cells in asthma

doi: 10.1111/j.1365-2249.2010.04161.x

Figure Lengend Snippet: (a) Scatter-plot showing chemokine receptor homologous molecule expressed on T helper type 2 (Th2) lymphocytes (CRTH2) expression on (♦) interferon (IFN)-γ + , (•) interleukin (IL)-4 + and (▴) IL-13 + T cells derived from peripheral blood mononuclear cells (PBMC) from asthmatic subjects. Cells were stimulated for 6 h with phorbol myristate acetate (PMA) and calcium ionophore. *** P < 0·001 using the Mann–Whitney U -test. Bars represent mean percentages. (b) Scatter-plot showing CRTH2 expression on (♦) IFN-γ + , (•) IL-4 + and (▴) IL-13 + T cells derived from PBMC from normal subjects. Cells were stimulated for 6 h with PMA and calcium ionophore. *** P < 0·001 using the Mann–Whitney U -test. Bars represent mean percentages.

Article Snippet: PBMC and BAL cells were incubated with biotinylated anti-CRTH2 mAb (Mitenyi Biotec, Bergisch Gladbach, Germany) at 4°C while suspended in PBS and 0·5% BSA.

Techniques: Expressing, Derivative Assay, MANN-WHITNEY

Journal: Clinical and Experimental Immunology

Article Title: CRTH2 expression on T cells in asthma

doi: 10.1111/j.1365-2249.2010.04161.x

Figure Lengend Snippet: (a) Chemokine receptor homologous molecule expressed on T helper type 2 (Th2) lymphocytes (CRTH2) + T cells in bronchoalveolar lavage (BAL) from asthmatic (•) and normal (▴) subjects. There was a significant difference between asthmatic and normal controls. * P < 0·05 using the Mann–Whitney U -test. Bars represent mean percentages. (b) CRTH2 + T cells in paired blood (□) and BAL (▵) samples from normal subjects. Lines connect samples from the same subject. * P < 0·05 using the Mann–Whitney U -test.

Article Snippet: PBMC and BAL cells were incubated with biotinylated anti-CRTH2 mAb (Mitenyi Biotec, Bergisch Gladbach, Germany) at 4°C while suspended in PBS and 0·5% BSA.

Techniques: MANN-WHITNEY

Journal: Clinical and Experimental Immunology

Article Title: CRTH2 expression on T cells in asthma

doi: 10.1111/j.1365-2249.2010.04161.x

Figure Lengend Snippet: Scatter-plot of the percentage of chemokine receptor homologous molecule expressed on T helper type 2 (Th2) lymphocytes (CRTH2) + bronchoalveolar lavage (BAL) T cells from asthmatic subjects expressing interferon (IFN)-γ (♦), interleukin (IL)-4 (•) and IL-13 (▴). ** P < 0·01 using the Mann–Whitney U -test. Bars represent mean percentages.

Article Snippet: PBMC and BAL cells were incubated with biotinylated anti-CRTH2 mAb (Mitenyi Biotec, Bergisch Gladbach, Germany) at 4°C while suspended in PBS and 0·5% BSA.

Techniques: Expressing, MANN-WHITNEY